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Objective To analyze the expression of muscle scinderin(SCIN)in primary hepatocellular carci-noma(HCC)with portal vein tumor thrombus (PVTT)and its clinical significance.Methods 46 primary HCC patients with PVTT were selected.All patients'carcinoma adjacent tissues,HCC tissues,PVTT tissues were collected. SCIN expression in three kinds of surgical specimen was detected by immunohistochemical staining,the results were compared.All patients were followed up for three years,the recurrence rate within half a year and 3 -year mortality were compared.Results The positive expression rates of SCIN in carcinoma adjacent tissues,HCC tissues and PVTT tissues were 69.57%,45.65%,23.91%,which showed a decreasing trend.The relapsed within six months and 3 -year mortality rate of the SCIN -negative patients were 85.71%,95.24%,which were significantly higher than those of the SCIN -positive patients.The SCIN expression level was closely related to tumor size,with or without coating and alpha -fetoprotein level,the differences were statistically significant (χ2 =0.000,0.007,0.035,all P <0.05). Conclusion The SCIN expression level in primary HCC patients with PVTT is low,and it can be used as a prognos-tic indicator for primary HCC patients with PVTT.
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Objective To analyze the expressions of tumor stem cell markers Lgr5 and CD44 in esophageal carcinoma and its clinical pathological significance.Methods A total of 108 cases of patients with esophageal cancer, 35 cases of stage Ⅱ, 40 cases of stage Ⅲ, 4 cases of stage Ⅳ. After tumor specimens were stained, the expressions of Lgr5 and CD44 in esophageal cancer tissue were detected and its relationship with the clinical features were analyzed.Results There were relations between expressions of Lgr5 and CD44 in esophageal cancer tissue and clinical staging, the later the stageing, the higher expression rate of Lgr5 and CD44, but there was no significant differece with Chi-square test. The Spearman correlation analysis showed there was no correlation between Lgr5 or CD44 and esophageal cancer condition. Conclusion CD44 and Lgr5 are highly expressed in esophageal cancer tissue, but the correlation between CD44 and Lgr5 expression and esophageal cancer condition is uncertain.
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[ABSTRACT]OBJECTIVETo study the expressions of 34βE12, Galectin-3 and HBME-1 in thyroid nodules, and to explore its diagnostic value for papillary thyroid carcinoma (PTC).METHODSEn VisionTM immunohistochemical technique was used to detect the expression of 34βE12, Galectin-3 and HBME-1 in 352 thyroid lesions. The correlation between the expressions of the 3 protein markers and clinicopathological characteristics was evaluated. The receiver operating characteristic area under the curve (ROC-AUC) and their index for diagnosis evaluation were also calculated.RESULTSThe positive rates of 34βE12, Galectin-3 and HBME-1 in 246 PTC lesions were significantly higher than those in benign nodules (P<0.001). There was no relationship between the expression of the 3 protein markers and clinicopathological characteristics (eg. gender, age, numbers of lesions, tumor size, capsular invasion, lymph node metastasis, TNM staging). The ROC-AUC of 34βE12, Galectin-3 and HBME-1 for diagnosis of PTC was 0.936, 0.915 and 0.898 respectively. The sensitivity of 34βE12, Galectin-3 and HBME-1 for diagnosis of PTC was 94.3%, 95.5% and 91.1% respectively, while the specificity was 81.1%, 71.7% and 83.0% respectively, and the diagnostic accuracy rate was 90.3%, 88.4% and 88.6% respectively.CONCLUSION The expressions of 34βE12, Galectin-3 and HBME-1 are statistically different between PTC and benign lesions, but no associations are found with clinicopathological characteristics, indicating the three protein markers have important diagnostic value for PTC.